Ukukhanya emaphethelweni abomvu naseduze kwe-infrared ye-spectrum kusheshisa ukuphulukiswa kuwo wonke amaseli nezicubu. Enye yezindlela abakufeza ngayo lokhu ukusebenza njengama-antioxidants anamandla. Futhi kuvimbela ukukhiqizwa kwe-nitric oxide.
Ingabe ukukhanya okubomvu nokucishe kube yi-infrared kungavimbela noma kubuyisele emuva ukulahlekelwa ukuzwa?
Esifundweni sango-2016, abacwaningi basebenzise ukukhanya okuseduze kwe-infrared kumaseli okuzwa ngaphakathi kwe-vitro ngaphambi kokuwabeka ngaphansi kokucindezeleka kwe-oxidative ngokuwabeka ezifweni ezihlukahlukene zobuthi. Ngemva kokubeka amaseli alungisiwe esimweni sawo ebuthini be-chemotherapy kanye ne-endotoxin, abacwaningi bocwaningo bathole ukuthi ukukhanya kushintshe indlela umzimba we-mitochondrial osebenza ngayo kanye nempendulo yokucindezeleka kwe-oxidative isikhathi esingafika emahoreni angama-24 ngemva kokwelashwa.
“Sibika ukwehla kwama-cytokine okuvuvukala kanye namazinga okucindezeleka avela ku-NIR esetshenziswa kumaseli okuzwa e-HEI-OC1 ngaphambi kokwelashwa nge-gentamicin noma i-lipopolysaccharide,” kubhala abalobi besifundo.
Imiphumela yocwaningo ikhombisile ukuthi ukwelashwa kwangaphambi kokwelashwa ngokukhanya okuseduze kwe-infrared kunciphisa izimpawu zokuvuvukala ezihambisana nokwanda kwezinhlobo ze-oxygen ezisabelayo kanye ne-nitric oxide.
Isifundo #1: Ingabe Ukukhanya Okubomvu Kungaguqula Ukulahlekelwa Yizindlebe?
Umphumela wokukhanya okuseduze kwe-infrared ekulahlekelweni kokuzwa ngemva kobuthi be-chemotherapy uhlolwe. Ukuzwa kuhlolwe ngemva kokunikezwa i-gentamicin futhi kwaphinde kwahlolwa ngemva kwezinsuku eziyi-10 zokwelashwa kokukhanya.
Ekuskeni izithombe ze-electron microscopic, "i-LLLT yandise kakhulu inani lamaseli ezinwele emaphendulweni aphakathi naphansi. Ukuzwa kwathuthukiswa kakhulu yi-laser irradiation. Ngemuva kokwelashwa kwe-LLLT, kokubili umkhawulo wokuzwa kanye nenani lamaseli ezinwele lathuthuka kakhulu."
Isifundo #2: Ingabe Ukukhanya Okubomvu Kungaguqula Ukulahlekelwa Yizindlebe?
Kulolu cwaningo, amagundane athola umsindo omkhulu ezindlebeni zombili. Ngemva kwalokho, izindlebe zawo zangakwesokudla zashiswa ngokukhanya okucishe kube yi-infrared imizuzu engama-30 ukwelashwa nsuku zonke izinsuku ezi-5.
Ukulinganiswa kwempendulo yesimiso sobuchopho sokuzwa kwembula ukululama okusheshayo komsebenzi wokuzwa emaqenjini aphathwe nge-LLLT uma kuqhathaniswa neqembu elingalashwanga ezinsukwini 2, 4, 7 kanye no-14 ngemva kokuchayeka emsindweni. Ukuhlolwa kwesimo somzimba kwembula nesilinganiso sokusinda kwamaseli ezinwele zangaphandle esiphezulu kakhulu emaqenjini e-LLLT.
Befuna izinkomba zokucindezeleka kwe-oxidative kanye ne-apoptosis kumaseli angelashwanga uma kuqhathaniswa nalawa alashwa, abacwaningi bathole ukuthi “Kubonakale ukusebenza okunamandla kwamasosha omzimba ezicutshini zendlebe zangaphakathi zeqembu elingalashwanga, kanti lezi zimpawu zehlisiwe eqenjini le-LLLT ku-165mW/cm(2) amandla amakhulu.”
"Okutholakele kwethu kusikisela ukuthi i-LLLT inemiphumela yokuvikela i-NIHL ngokuvimbela ukubonakaliswa kwe-iNOS kanye ne-apoptosis."
Isifundo #3: Ingabe Ukukhanya Okubomvu Kungaguqula Ukulahlekelwa Yizindlebe?
Esifundweni sango-2012, amagundane ayisishiyagalolunye athola umsindo omkhulu futhi kwahlolwa ukusetshenziswa kokukhanya okuseduze kwe-infrared ekuvuseleleni ukuzwa. Ngosuku olulandelayo ngemuva kokuvezwa komsindo omkhulu, izindlebe zesobunxele zamagundane zaphathwa ngokukhanya okuseduze kwe-infrared imizuzu engama-60 izinsuku eziyi-12 zilandelana. Izindlebe zesokudla azizange zilashwe futhi zabhekwa njengeqembu lokulawula.
“Ngemuva kokukhanya kwemisebe kwe-12, umkhawulo wokuzwa wawuphansi kakhulu ezindlebeni zesobunxele uma kuqhathaniswa nezindlebe zesokudla.” Lapho kubhekwa kusetshenziswa i-electron microscope, inani lamaseli ezinwele ezizwayo ezindlebeni eziphathwe lalilikhulu kakhulu kunelezindlebe ezingelashwanga.
"Okutholakele kwethu kusikisela ukuthi ukushiswa ngemisebe ye-laser esezingeni eliphansi kukhuthaza ukululama kwemingcele yokuzwa ngemva kokulimala okukhulu kwe-acoustic."